Glycerol Quantification Assay

GLYCEROL [GLYCERIN or GLYCERINE, C3H5(OH)3] is widely used in foods, beverages and pharma-ceutical formulations. It is also a main byproduct of biodiesel production. Simple, direct and automation-ready procedures for measuring glycerol concentrations find wide applications. W2 Optronics’s glycerol assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase and color reactions in one step. The fluorescence intensity at ex/em = 530/590nm is directly proportional to glycerol concentration in the sample measured by FluoroQuik fluorometer.

Part Number: EGLY-200

Specifications

  • Sample: Serum, plasma, and other biological samples.
  • Assay Performance:
    • Sufficient for about 200 tests.
    • Sensitive and accurate. Use as little as 10 µL samples.
    • Linear detection range: 2 -100 µM, 18 - 920 µg/dL, or 0.18 - 9.20 ppm.
    • Simple and convenient. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature.
  • Shipping and storage: the kit is shipped on ice. Store Assay Buffer at 4°C and other reagents at -20°C. Shelf life of 12 months after receipt.

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